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Lab handling guides

Practical notes on storing, handling and documenting lyophilized peptides in the lab.

Storage

Lyophilized peptides are stable when kept cold, dry and dark.

  • Long term: −20 °C in the sealed vial, inside its box or an opaque container.
  • Short term: 2–8 °C is acceptable for a few weeks.
  • Avoid: frost-free freezers, direct light, and repeated temperature swings.
  • Solutions: keep at 2–8 °C, or split into single-use aliquots before freezing to avoid freeze–thaw cycles.

Reconstitution technique

General bench technique for dissolving a lyophilized peptide. Choose the diluent and concentration according to your own validated protocol.

  1. Let the vial reach room temperature before opening to prevent condensation.
  2. Wipe the rubber septum with 70% isopropyl alcohol and let it dry.
  3. Add the diluent slowly, running it down the inside wall of the vial rather than onto the powder.
  4. Swirl gently or roll the vial until dissolved. Don't shake, which can cause foaming and aggregation.
  5. Label the vial with the date, concentration and lot code.

Some hydrophobic peptides need a small amount of a co-solvent first. Check the literature for your compound.

Reading a COA

Check three things: the lot code matches your vial, the testing lab is named and independent, and the report includes the chromatogram or mass spectrum rather than just a number. Our COA article goes line by line.

Receiving an order

  • Open the parcel promptly and check every vial against your packing list.
  • Confirm the crimp seals and flip-off caps are intact.
  • Record each lot code in your inventory.
  • Move vials to cold storage the same day.
  • Report any damage or discrepancy within 48 hours with photos, as described in our returns policy.

Lab safety & disposal

  • Handle all compounds with gloves, eye protection and a lab coat.
  • Work in a clean area and use aseptic technique when preparing solutions.
  • Dispose of sharps in an approved sharps container.
  • Dispose of unused compounds and solutions according to your institution's chemical waste procedures and local regulations.